tapi 1 Search Results


tapi 1  (MedChemExpress)
94
MedChemExpress tapi 1
Tapi 1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
94/100 stars
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tapi 1  (Bio-Techne corporation)
94
Bio-Techne corporation tapi 1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
94/100 stars
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tapi 1  (Biosynth Carbosynth)
92
Biosynth Carbosynth tapi 1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
92/100 stars
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tapi 1  (Selleck Chemicals)
93
Selleck Chemicals tapi 1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tapi 1/product/Selleck Chemicals
Average 93 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
93/100 stars
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tapi 1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tapi 1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tapi 1/product/Santa Cruz Biotechnology
Average 93 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
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inhibitor tapi  (Biosynth Carbosynth)
90
Biosynth Carbosynth inhibitor tapi
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Inhibitor Tapi, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
inhibitor tapi - by Bioz Stars, 2026-03
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metalloproteinase inhibitor inh3855 pi  (Biosynth Carbosynth)
90
Biosynth Carbosynth metalloproteinase inhibitor inh3855 pi
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Metalloproteinase Inhibitor Inh3855 Pi, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/metalloproteinase inhibitor inh3855 pi/product/Biosynth Carbosynth
Average 90 stars, based on 1 article reviews
metalloproteinase inhibitor inh3855 pi - by Bioz Stars, 2026-03
90/100 stars
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culture medium  (Tocris)
93
Tocris culture medium
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Culture Medium, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
culture medium - by Bioz Stars, 2026-03
93/100 stars
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tapi 1  (TargetMol)
94
TargetMol tapi 1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tapi 1/product/TargetMol
Average 94 stars, based on 1 article reviews
tapi 1 - by Bioz Stars, 2026-03
94/100 stars
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tapi-1  (Enzo Biochem)
90
Enzo Biochem tapi-1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tapi-1/product/Enzo Biochem
Average 90 stars, based on 1 article reviews
tapi-1 - by Bioz Stars, 2026-03
90/100 stars
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tapi-1  (Biomol GmbH)
90
Biomol GmbH tapi-1
(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM <t>TAPI-1</t> (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).
Tapi 1, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tapi-1/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
tapi-1 - by Bioz Stars, 2026-03
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Image Search Results


(A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM TAPI-1 (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).

Journal: Oncotarget

Article Title: The enhanced susceptibility of ADAM-17 hypomorphic mice to DSS-induced colitis is not ameliorated by loss of RIPK3, revealing an unexpected function of ADAM-17 in necroptosis

doi: 10.18632/oncotarget.24410

Figure Lengend Snippet: (A) MEF from WT and ADAM17 ex/ex mice were left untreated or preincubated for 30 min with 20 μM zVAD-fmk in the absence or presence of 1 μg/ml CHX, followed by stimulation with 100 ng/ml TNF. After 16 h, loss of membrane integrity was determined as a marker for cell death by flow cytometric analysis of PI-positive cells. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. (B) in parallel, cell lysates were analyzed by Western blot for presence of pMLKL, MLKL, pRIPK3, RIPK3, cleaved caspase-3 and pBcl-2. Detection of actin served as a loading control. (C) WT MEF were left untreated or preincubated for 30 min with 3 μM GW280264X (GW), 3 μM GI254023X (GI), 10 μM marimastat (M) or 50 μM TAPI-1 (T) in combination with zVAD-fmk, CHX and subsequent addition of TNF as in A before cell death was analyzed. Each measurement represents the mean of three parallel determinations, error bars indicate the corresponding SD. ns, not significant, * , p<0.05, ** , p<0.01, *** , p<0.001 (two-tailed unpaired Student’s t -test).

Article Snippet: Highly purified human recombinant TNF was provided by BASF Bioresearch (Ludwigshafen, Germany). zVAD-fmk was from Bachem (Bubendorf, Switzerland), GW280264X from Iris Biotech (Marktredwitz, Germany), marimastat from BioTechne and GI254023X and TAPI-1 from Merck.

Techniques: Membrane, Marker, Western Blot, Two Tailed Test